Dear Jeff: The sequence here is NYT. I think it is glycosylated here, isn't it? Thanks a lot.
On Jan 8, 2008 11:37 AM, Jeff Lee <[EMAIL PROTECTED]> wrote: > Hi, > Do you know what is the protein sequence in this region. If it is > glycosylated, it should have a distinctive motif (N-X-S/T). You might have > the first NAG attached to the Asn if the motif is there. > > Jeff > > > On Jan 7, 2008, at 7:19 PM, Jiamu Du wrote: > > Dear All: > I am refining a structure at 2.6 A reslution. The protein is expressed in > CHO cell, so it might be glycosylated. > While refining an Asn residue as shown in the figure of the attachment, I > found some strange density extended beyond the side chain of Asn. The sigma > level for the fofc map of the figure in the attachment is as high as 3.0. > My protein buffer is Tris and NaCl, and the reservior is PEG and NH4Cl. I > believe this is not water mlecules. Considering the residue is Asn, I guess > it might be carbohydrate. > Is this density like a carbohydrate molecule? How to model this density? > Thanks and Happy New Year. > > -- > Jiamu Du > State Key Laboratory of Molecular Biology > Institute of Biochemistry and Cell Biology Shanghai Institutes for > Biological Sciences > Chinese Academy of Sciences (CAS)<asn.JPG> > > > -- Jiamu Du State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences (CAS)
