I doubt if you should ever t hrow away data if the I/SigI is sensible in the outer shell.
Have you tried TLS refinement or loosened the B restraints? Often higher Rfree at higher resolution are related to B refinement problems. Eleanor 杨柳青 wrote: > Hi,dear all: > I try to refine one protein structure using refmac5,now I got some > problems. > For example,first,the resolution is 2.25,R-free is 30,completeness is 90%. > Then I changed resolution to 2.35,R-free became 28.0,completeness > is 91%. > When I changed resolution to 2.45,R-free became 27.5,completeness > is 92%. > So,when resolution from 2.25 to 2.35,R-free goes down a lot,but > resolution from 2.35 to 2.45,R-free nearly the same,is that meaning > resolution should around 2.35? Should I change resolution during refinement? > And is there any good software for refinement? > Thanks a lot! > Best wishes! > liuqing > > _________________________________________________________________ > 天凉了，添衣了，心动了，“七件”了 > http://get.live.cn >
