Did you grow Malic Acid crystals?
I see no reason why glycerol cannot be the precipitant. I am aware that
at least one protein is crystallized with ethylene glycol as the
precipitant.
On Wed, 9 Jan 2008, JINJIN ZHANG wrote:
Dear All,
I have got large and nice crystals in a condition that contains 40%
glycerol, 75 mM DL-Malic Acid pH7.0, and that's it! No precipitant?! No
buffer?! I'm so comfused. As far as from the product website, the Malic
Acid has no buffering function. I don't know whether it's a precipitant.
The protein itself was stored in a buffer contains 20mM Tris pH7.5, 150mM
NaCl and 10% glycerol. I'm having difficulties in getting heavy metal
bound derivatives. I was wondering whether the Malic Acid interferes
heavy metal binding? Does anyone has any success with crystals grow with
similar conditions? Any comment and suggestion is highly appreciated.
Regards,
Jinjin Zhang
