Thank you all for the quick replies - I'm trying many of the tips in small batches and would send out a feedback as soon as appropriate, gratefully, - J.J.
On 2/14/08, Jacob Wong <[EMAIL PROTECTED]> wrote: > > Dear all, > > I just ran into this problem and would like to see if I could get some > helpful tips before my protein completely crashes out. > > I have a protein as 6His fusion and it remained bound to the Ni resin with > 40 mM Imidazole wash (added to 1XPBS) but then was eluted off with 200 mM > (added to 1XPBS). The protein seemed to be highly concentrated in the > elution and began to get cloudy right away, with more and more precipitation > produced over a matter of minutes. I felt so helpless, didn't know what to > do, and then decided to add 5% of glycerol into one of the fractions but > that made it even more cloudy (ohh no...). > > > While the protein is dying in the tube, do you have some quick remedy for > me? Thanks very much, -J.J. >
