Dear all,
I am with the same problem of Jacob.
My protein is
precipitating, especially when I 'freeze it'. I am using a His Trap HP column
coupled with a desalting column. Already tried to elute with gradient of
imidazole (that allowed an elution with less amount of imidazole), but
even then, the protein still crashes-out.
I am trying to use the
protein immediately after purified it, but this is not always possible
and I am losing much protein with that.
I will try to use the tips that have already been said.
But if someone can help me more, I will thank!!!
Thanks a lot!
Juliana
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