Dear all;
I am a fresher of Refmac5. And now I'm trying to refine a protein and labelled
RNA complex using Refmac. It's a Iodo-U labelled RNA. Unfortunately, these is
no appropriate lib file in the current refmac dictionaries. So I use cif file
which created by Refmc itself, surprisingly, the base of uridine distorted
greatly. Could someone like to tell me how to make the appropriate cif file to
solve this problem. Another question is that the RNA has a phosphate group in
5', How to define this in the cif file?
Could someone help me? Thanks a lot!
Best wishes,
Hai-Feng Hou
Biological macromolecule group
Beijing Synchrotron Radiation Facility
Institute of High Energy Physics
Chinese Academy of Sciences
Beijing, China
Tel: +86-10-88234208
E-mail: [EMAIL PROTECTED]
2008-02-18
