I have not tried to make 1M solutions of the indicated AA, but i have had good luck of using what they suggest in that specific paper. By using 50mM of each (Glu and Arg) I was able to increase the solubility of a 44kDa protein (a Serpin) from ~1mg/ml to above 17mg/ml. So in my case it really worked. This was for the purpose of doing liquid state NMR on that protein. I does help to solubilize the two aa in 1:1 ratio as the two combined improve their individual solubility.
Jan Jensen On Thu, February 28, 2008 4:33 pm, Paul Paukstelis wrote: > posting for a colleague: > > In Golovanov et al. 2004 they indicate it is possible to make a 1M > solution of free amino acids (not amino acid salts) Glu+Arg in the > context of generating solutions for keeping proteins soluble. Has anyone > been able to do this? > >
