Hi,
I have a protein with two independently folded domains. I can express either
one in bacteria with pretty good expression yield. However, when I put them
together with a linker, the expression drops significantly. I can barely see
any soluble protein and most of it is now inclusion bodies. I used the same
bacteria strain and expression/purification conditions. Is it normal? Any
suggestion about how to improve? Many thanks.
Best,
Chen
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