Hi all, I have a question about registering Se. Since diffraction quality of my SeMet dataset is better than a native one, I want to use a SeMet dataset (collected at a remote wavelength) for a final model while I am trying to get a decent native dataset. I tried to put MSE in some MET positions based on Fo-Fc density, but this caused an increase in R free factor after refinement (about 2 %). I'm using a Coot for model building and Refmac for refinement. If anyone has experienced to refine SeMet data, I hope to share some tips. Thanks, Eunyoung
