Lower temperature, use chaperones (e.g. TAKARA set), refolding?
Quoting shivesh kumar <[EMAIL PROTECTED]>:
Dear all, Sorry for the off-topic question... What can be done to avoid a protein going inside inclusion body.The gene is cloned in pET30a with C-ter his tag and expressed in BL21-DE3 from 37 to 18C for 3-4 hr with .5mM of IPTG,it is going to inclusion body.All suggestions are welcome. Thanx in advance. Shivesh
