Hi all,
I have some crystals from a condition with a high concentration of
LiCl (3.5M is a need). The crystals look pretty, however I had no
luck because they diffract very poorly (~8Å). Dehydration pushes
resolution to ~4 Å, however, it damages the crystal lattice and makes
disorder especially in one direction. One thing I learned is that
LiCl releases a huge dilution heat at high concentration, to which my
crystal seems to be very sensitive. Does anyone have some successful
experience in crystallization and cryo with LiCl at such a high
concentration like >3.0 M? Thanks a lot.
Lijun Liu, PhD
Institute of Molecular Biology
HHMI & Department of Physics
University of Oregon
Eugene, OR 97403
541-346-4080
