This reminded me of a related article I came across a while back about controlling humidity with saturated solutions.
# Saturated Solutions For the Control of Humidity in Biological Research # Paul W. Winston and Donald H. Bates # Ecology, Vol. 41, No. 1 (Jan., 1960), pp. 232-237 Hope that helps Tom ** Tom Walter B.Sc. M.Res. ** ** Oxford Protein Production Facility Tel: +44 (0)1865 287747 ** ** Wellcome Trust Centre for Human Genetics Fax: +44 (0)1865 287547 ** ** Roosevelt Drive [EMAIL PROTECTED] ** ** Headington, Oxford OX3 7BN http://www.oppf.ox.ac.uk ** ---- Original message ---- >Date: Tue, 8 Apr 2008 13:42:21 +0200 >From: Kay Diederichs <[EMAIL PROTECTED]> >Subject: Re: [ccp4bb] which concentrated salt has lowest vapour pressure? >To: [email protected] > >Dear all, > >once again this bulletin board proved to be a great ressouce. I obtained >15 emails since I posted - thanks to everybody! > >I composed a summary for the CCP4 wiki, which can be found at: ><http://strucbio.biologie.uni-konstanz.de/ccp4wiki/index.php/Crystallization_screens_and_methods#Desiccation_of_an_existing_screen_which_shows_no_sign_of_crystallization_or_precipitation> > >thanks again, > >Kay > >Kay Diederichs schrieb: >> Dear all, >> >> a protein which we work on is available in low quantity. The only >> crystallization screen we set up is completely clear, no precipitate, >> nothing. >> >> Now we would like to modify the reservoirs of this screen, by adding >> LiCl or Ammoniumsulfate or ... , with the goal of reducing the vapour >> pressure, to at least get the protein concentration in the drop into the >> range where "something happens". >> >> Does anyone have advice as to which salt we should add (to the reservoir >> only)? AmSO4 is only soluble to 4M, LiCl goes to 10M. But vapour >> pressure reduction is not the same as molarity. >> >> thanks for any insight, >> >> Kay > > >-- >Kay Diederichs http://strucbio.biologie.uni-konstanz.de >email: [EMAIL PROTECTED] Tel +49 7531 88 4049 Fax 3183 >Fachbereich Biologie, Universität Konstanz, Box M647, D-78457 Konstanz >________________ >smime.p7s (5k bytes)
