Dear Jim,

The funny part is that the same journals will publish NMR structures.  In
terms of data/parameter ratio or in terms of comparing quality
(Battacharya, Tejero and Montelione, Proteins:structure function genetics
66:778-795), the  good NMR structures are about 2.5A resolution and most
NMR structures are around 3.5A resolution.

I do not think the resolution is the problem, I think poor review is the
problem.   There are X-ray structures to 2.5A resolution that are worse
than those at 3.5A resolution in the PDB.....

Rams.



> Dear Jim,
>
> I guess you entered the ABC transporter zone.
>
> You say you use the structure to show the movement of helices.
> One important point is whether this structure is based on a
> molecular replacement solution, or it was built from experimental
> phases. There are numerous cases where adding some experimental
> phasing helped to convince the editors.
>
> Good luck,
>
> Rob Meijers
> Synchrotron Soleil
>
> Jim Naismith <[EMAIL PROTECTED]> wrote: Dear All,
>   I have an interesting problem, we have a 3.45A structure of
> a membrane protein. We have just been told that the structure is "too low
> resolution to be considered as the uncertainty is too high". We use the
> structure to identify helices which have moved.
>
> Is there a blanket ban on low res structure operating at the moment?
>
> The structure was refined extremely tightly, MolPROB 98th centile. (I will
> happily send the data and structure to anyone who wishes to validate.) The
> editors simply ignored everything but the res limit (I/sI in the last
> shell
> was 1.8 with a redundancy of 4)
>
> Of course we will begin the usual journal shopping. However, does anyone
> know how to convince editors and non-xtallographers that 3.45A is valid?
>
> Best
> Jim
>
>
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