Actually, 3.5mins plate-to-plate for our 3-drop protocols (50:100,
75:75, 100:50ul) on our mosquito. (Not sure what protocol Artem is
using, must have lots of tip changes.)
Janet's reply reads slightly misleadingly: if you're concerned with
plates/hour (which we are, since we have users show up with piles of
20-40 trays on Friday evenings), then mosquito seems(*) to be quickest
by quite a bit, as it does not have a wash cycle: as soon as one plate
is done, you wang on the next one.
If you only do a plate at a time(**), then the wash cycle won't bother
you, and phoenix is indeed as quick as mosquito.
phx.
(*) I must confess, though, I've not done extensive research recently.
When I did poke around a year ago, the plate-to-plate timing was
exceedingly hard to get out of sales-persons -- frustratingly.
(**) Mind you, I'm not sure why you'd only do a plate at a time: protein
variation is far more useful to explore than chemical variation.
Artem Evdokimov wrote:
About 2 – 2.5 minutes on Mosquito for single-drop protocol, scaled
according to the number of drops per well (7 minutes for 3-drop
trays). About 25 minutes on a Tecan-derived platform (numbers vary
greatly depending on the particular configuration).
Artem
------------------------------------------------------------------------
*From:* CCP4 bulletin board [mailto:[EMAIL PROTECTED] *On Behalf
Of *JOE CRYSTAL
*Sent:* Monday, April 14, 2008 5:10 PM
*To:* [email protected]
*Subject:* Re: [ccp4bb] Fwd: [ccp4bb] crystallisation robot
Hi,
Does anyone have information about how long it takes to set up a
96-well tray for the crystallization robots available? Besides cost
per tray and maintenance cost, another important feature we consider
is the time for setting up a 96-well tray. It is an important factor
since we are talking about sub-microliter drops.
Best,
Joe
On Fri, Jan 18, 2008 at 12:28 PM, Lisa A Nagy <[EMAIL PROTECTED]
<mailto:[EMAIL PROTECTED]>> wrote:
Al's Oil on the plates:
What a nightmare!!!!!!!
The oil creeps up the plate and over the sides. It dissolves adhesives.
It makes me say bad words in multiple languages.
Bigger drops + no oil = fewer bad words.
Lisa
--
Lisa A. Nagy, Ph.D.
University of Alabama-Birmingham
[EMAIL PROTECTED] <mailto:[EMAIL PROTECTED]>
-----Original Message-----
From: CCP4 bulletin board [mailto:[email protected]
<mailto:[email protected]>] On Behalf Of
Patrick Shaw Stewart
Sent: Friday, January 18, 2008 2:20 AM
To: [email protected] <mailto:[email protected]>
Subject: [ccp4bb] Fwd: [ccp4bb] crystallisation robot
One thing that people often overlook is that quite a lot of protein
can be lost by denaturation on the surface of the drop. This is more
significant for smaller drops. Two suggestions: (1) increase the
proportion of protein in the - technical term - teeny drop to say two
thirds and (2) cover the drops with oil eg Al's oils
(silicone/paraffin). You still get vapor diffusion though the oil ,
and you'd like to slow up equilibration. of course (2) slows up the
robotics a little, but both should be trivial to set up..
