Hi Junyu
It is possible at 6 Å resolution, what I would try is to cut your data
and try searching in different resolution ranges 20-7 or 25-6.5 , if you
are uncertain about your search model, try with a higher RMS value try
going up to 2.0. If you have any data, even at very low resolution, with
heavy atom soaks try to see if you can locate your heavy atom scatterers
in the anomalous difference Fourier map using your MR model phases.
Wrong space group, incomplete data, poor search models and ....low
resolution :-) will make your life hard. Going back to the lab and try
getting some experimental phases and better crystals, is probably where
most of your time should be spend at this stage.
good luck
Preben
Junyu Xiao wrote:
Hi,
Does anyone have experience with molecular replacement at low
resolution? I have a 6Å dataset with probably 3-4 monomers per ASU.
Phaser doesn't seem to give me clear results. Can I get some advice?
Any comment will be appreciated.
Thanks a lot,
Junyu
=================================
Junyu Xiao
Department of Biological Chemistry,
University of Michigan
Lab address:
3163 Life Sciences Institute,
University of Michigan,
210 Washtenaw Avenue
Ann Arbor, MI, 48109-2216
Phone: 734-615-2078
==================================
--
Jens Preben Morth, Ph.D
Aarhus University
Department of Molecular Biology
Gustav Wieds Vej 10 C
DK - 8000 Aarhus C
Tel. +45 8942 5025, Fax. +45 8612 3178
[EMAIL PROTECTED]
website: http://person.au.dk/da/[EMAIL PROTECTED]
