Hi Mike,
how does your protein look on a sizing column ? Perhaps a dimer ? That
would then explain the tendency to form dimers in different crystal
forms, as it is a biological relevant dimer. for the eventuality that
you have no clue how the sizing looks like, run one and find out.
Juergen
Michael Colaneri wrote:
Dear colleagues,
I have a structure with two identical dimers per asymmetric unit. If
the dimers are identical in different crystalline environments and in
different crystal forms they should be particularly stable.
How does such a stable dimer crystallize? Is it necessay to pre-exist
in the specific conformation in solution (even to a lesser extent than
a major monomeric form) or a very stable dimer can assemble from the
very beginning durnig crystallization? And can the same dimer form
during crystallization in different crystalline environments without
it being present in solution to any (even infinitesimal) extent?
I would appreciate all responses.
Thanks.
Mike Colaneri
--
Jürgen Bosch
University of Washington
Dept. of Biochemistry, K-426
1705 NE Pacific Street
Seattle, WA 98195
Box 357742
Phone: +1-206-616-4510
FAX: +1-206-685-7002
Web: http://faculty.washington.edu/jbosch
