This may be obvious, but I would run a size-exclusion column on the complex first. This will give you an idea as to how well these two proteins "stick" together.
On Wed, May 14, 2008 at 4:34 AM, Maarten Dewilde < [EMAIL PROTECTED]> wrote: > Dear CCP4bb, > > > > Can anyone point me to good reviews or books which describe how to > crystallize protein-protein complexes (or anyone willing to share his/her > experience)? In particular I'm interested in: > > > > (1) which tests to perform on your complex before you even start > thinking about crystallization > > (2) what should you take in account when you start to crystallize (any > other than 'use the Radaev and Sun' screen) > > (3) how to increase the success rate of crystallization (cross linking? > co-expression?) > > (4) any alternative techniques when crystallization fails (any other > than NMR) > > > > I would be very grateful! > > > > Thanks in advance, > > > > Maarten > > > > > > > > > > __________________________ > > > > Maarten Dewilde - PhD student > > > > Laboratory for Pharmaceutical Biology > O&N2 - PB 824 - Herestraat 49 > 3000 Leuven > > > > [EMAIL PROTECTED] > tel: +32 16 323434 > fax: +32 16 323460 > > > > > > > Disclaimer: http://www.kuleuven.be/cwis/email_disclaimer.htm for more > information. > >
