Andy I guess it all depends on what temperature you mean by cryocooling. I was not aware at the Low et. al. paper - interesting early work. Low et. al. did not study reduction in radiation damage below -130C as they noticed a large increase in mosaicity occurring so abandoned this method. They did study the reduction in radiation damage down to -13C. The Blake & Phillips paper is a systematic study of radiation damage at room temperature (no cryo). References to "later early" work are given in the introduction section of the review by Garman and Owen (2005) Acta D 62, 32-47 http://journals.iucr.org/d/issues/2006/01/00/ba5085/index.html Regards Colin
________________________________ From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Andrew Torelli Sent: 20 May 2008 17:51 To: [email protected] Subject: [ccp4bb] Primary literature for cyrocooling to mitigate radiation damage To the CCP4 community, I believe I have identified 2 appropriate citations for the use of cryocooling to mitigate the effects of radiation damage during a diffraction experiment. The first study is one that I have access to: Low, B. W., Chen, C. C., Berger, J. E., Singman, L. & Pletcher, J. F. (1966). Proc. Natl Acad. Sci. USA, 56, 1746-1750. The second study is one that I cannot find text for: Blake, C. F. F. & Phillips, D. C. (1962). Biological Effects of Ionizing Radiation at the Molecular Level, pp. 183-191. Vienna: IAEA. Could someone point me in the right direction to find this text? Alternatively, are there other citations that people use for the general use of cryocooling crystals in X-ray crystallography? Thank you for your time, Regards, -Andy Torelli <DIV><FONT size="1" color="gray">This e-mail and any attachments may contain confidential, copyright and or privileged material, and are for the use of the intended addressee only. If you are not the intended addressee or an authorised recipient of the addressee please notify us of receipt by returning the e-mail and do not use, copy, retain, distribute or disclose the information in or attached to the e-mail. Any opinions expressed within this e-mail are those of the individual and not necessarily of Diamond Light Source Ltd. Diamond Light Source Ltd. cannot guarantee that this e-mail or any attachments are free from viruses and we cannot accept liability for any damage which you may sustain as a result of software viruses which may be transmitted in or with the message. Diamond Light Source Limited (company no. 4375679). Registered in England and Wales with its registered office at Diamond House, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom </FONT></DIV>
