Dear All I am processing data from a crystal for a large macromolecular complex with mosflm. Cell dimensions are around 120 x 150 x 650, with a p222 spacegroup. To avoid overlaps, we have collected data with a oscillation of 0.1 degrees.
When I try to process the data with mosflm, mosaicity decreases along the processing to a very low values (0.05 to 0.1 depending on the images). The result is that I miss a lot of spots from the images. I would appreciate any help regarding : 1.- What's is the best strategy to process such a dataset ? 2.- Which are the critical parameters in mosflm to avoid these problems, and how to modify them ? 3.- and finally, can I use this data or your advice is to try to get crystals in a different spacegroup ? Many thanks Best regards Francisco ----------------------------------------- Francisco J. Enguita, Ph.D. Macromolecular Crystallography Laboratory ITQB EAN, Av. da República 2781-901 Oeiras Portugal Phone : +351-21-4469663 Fax : +351-21-4433644 E-mail : [EMAIL PROTECTED] -----------------------------------------
