Dear Yong-fu, Why would you worry about insect expression systems if you already can secrete your constructs in mammalian cells? For such proteins, transient expression in HEK cells for example gives higher yields than baculo, is faster, cheaper, you can nicely control glycosylation, easily do Se-Met labelling and so on.
Here are some references (PMID): 17355862, 17001101, 16823037, 11788735, 16082028. Radu ------------------------------------------ A. Radu Aricescu, PhD University of Oxford Wellcome Trust Centre for Human Genetics Division of Structural Biology Roosevelt Drive, Oxford OX3 7BN United Kingdom Phone: +44-1865-287551 Fax: +44-1865-287547 ---- Original message ---- >Date: Wed, 28 May 2008 10:40:16 -0400 >From: Yong-Fu Li <[EMAIL PROTECTED]> >Subject: [ccp4bb] insect expression system >To: CCP4BB@JISCMAIL.AC.UK > > Hi, > > I searched my mail box for possible answers you have > given but couldn't find any. The question is about > selecting insect expression systems for mammalian or > viral glycosylated proteins. There is confirmed > expression of the target proteins in mammalian > cells. They are secreted into the media. > > 1) Baculovirus system > 2) Drosophila system > > Which system would you recommend for high expression > and convenience? > Has anyone ever compared those systems side by side? > > Any suggestions or references are greatly > appreciated. > > Yongfu Li
