Try the program disulfide by design: http://www.ehscenter.org/dbd/ Its easy to install (at least on windows) and to run. You put in a PDB file and the program will look in the structure where disulphide bonds are possible. It also ranks the candidates in energies. Finally, it can generate a PDB file with a particular disulphide. Quite a nice program.
Bert van den Berg University of Massachusetts Medical School Program in Molecular Medicine Biotech II, 373 Plantation Street, Suite 115 Worcester MA 01605 Phone: 508 856 1201 (office); 508 856 1211 (lab) e-mail: [EMAIL PROTECTED] http://www.umassmed.edu/pmm/faculty/vandenberg.cfm -----Original Message----- From: CCP4 bulletin board on behalf of amit sharma Sent: Thu 5/29/2008 11:03 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] mutation to cysteines Dear All, Sorry for a non-CCP4 question. I intend to mutate a couple of residues to cysteines(so that they form a disulphide linkage) in a certain region of my protein. Could I please be directed to program(s) that would reliably let me do that, prior to primer designing? Thanks in advance, Amit
