Dear All,
I am refining a structure with 2.5A resolution by refmac5. I
could find the solution by MR using molrep. After fitting the model, I
refined the structure again with 0.3 weighting term, but the output PDB file
shows many splits in the residues. So I used 'auto' as a weighting term,
then I didn't find any splits. At the same time I noticed that figure of
merits is not increasing after model fitting. But the correlation
coefficient is more than 80%. Also while refining with auto weighting term,
the figure of merit goes down about 25% than previous.
Finally I find that some of the residues show high B factor. Could
anyone give suggestions to reduce the B-factor?
Thanks in advance!
Yours sincerely,
Sampath
