You can coexpress the enzyme with chaperone (e.g GroEL and GroES)....very often this is very successful. (There are bunches of ways though...)
Best wishes, Arundhati 2008/6/23 yangliuqing <[EMAIL PROTECTED]>: > Hello,everyone! > Sorry to bother you! > Now I have an enzyme cloned in pQE2 ,expressed in E.Coli M15 at room > temperature,but there are a lot of inclusion bodies,perhaps 90%,it is a big > trouble because I need to get much enzyme. > Is there anyone knows how to solve? > Thank you very much! > Best wishes! > liuqing > > ------------------------------ > Windows Live Writer，支持离线撰写博客内容，随时随地想写就写。 > 立即使用！<http://get.live.cn/product/writer.html> >
