dear john, the reference for this in my opinion is still the 1994 paper by george and wilson where they describe the use of the second virial coefficient parameter as a metric for quantifying the potential that certain conditions have to favour crystallisation. see for instance bonete and vivares, Acta Cryst. (2002). D58, 1571-1575. cheers, jose brandao
________________________________ From: CCP4 bulletin board on behalf of John Luz Sent: Mon 23/06/2008 21:14 To: [email protected] Subject: [ccp4bb] RI and MALS for membrane proteins Would like to know what experiences people have had using in line refractive index and multiangle light scattering detectors for membrane protein purification. Specifically, if the information gathered from such experiments has been useful in optimizing solution conditions for membrane protein preparations to be used in crystallization trials. Thanks in advance for any replies. j John Gately Luz Snr. Scientist Protein Biochemistry SGX Pharmaceuticals, Inc. San Diego, CA phone: 858-228-1482 fax: 858-457-5362 email: [EMAIL PROTECTED] This email message is for the sole use of the intended recipient(s) and may contain confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message <DIV><FONT size="1" color="gray">This e-mail and any attachments may contain confidential, copyright and or privileged material, and are for the use of the intended addressee only. If you are not the intended addressee or an authorised recipient of the addressee please notify us of receipt by returning the e-mail and do not use, copy, retain, distribute or disclose the information in or attached to the e-mail. Any opinions expressed within this e-mail are those of the individual and not necessarily of Diamond Light Source Ltd. Diamond Light Source Ltd. cannot guarantee that this e-mail or any attachments are free from viruses and we cannot accept liability for any damage which you may sustain as a result of software viruses which may be transmitted in or with the message. Diamond Light Source Limited (company no. 4375679). Registered in England and Wales with its registered office at Diamond House, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom </FONT></DIV>
