Dear All, Sorry for off-topic question. Does anyone have any experience in purifying protein using pH gradient in Mono Q column?
I have been googling for a whole day, only one paper was found to mention performing pH gradient in Mono Q, but in a mixture of amine buffering species, which is a bit too complicated (J. Chromatogr. A 1164 (2007) 181 - 188. Can Tris-Cl/Tris-base or phosphate buffer give a linear pH gradient from pH 8.0 to 4.0? Is it usual to perform pH gradient in Mono Q as I don't want to ruin my Mono Q column... Any suggestions are welcome. Thanks in advance! Kind regards, Matt ---------------------------------------------------------------------------- Matthew LH Chu PhD Student School of Pharmacy and Pharmaceutical Sciences University of Manchester ----------------------------------------------------------------------------
