Hi everyone, can anyone tell me the protocol to revive human corneal cell lineor any cell line which requires serum free media
i usually used serum based media initially.....later switched it to serum free media for subculturing....only in case of human corneal cell line with the help of flask coated with fibronectin, BSA, and collagen..........but recently i m finding difficult to revive it....it deattaches after 1 or 2 days..what might be the reason? also we are unable to revive pRSV-T cell line using flask coating method. can anyone help on the same. thanks
