Hi Gina, From a lab near you ... gd resolvase without DNA: a hexagonal crystal form can be grown from either the catalytic domain only (missing the DNA binding domain, ~1/3 of the protein) or the intact protein. The binding domain is there when you run crystals down a gel, but disordered in the rather lousy, low-resolution, anisotropic electron density maps. Phoebe
Rice PA, Steitz TA. Model for a DNA-mediated synaptic complex suggested by crystal packing of gamma delta resolvase subunits. EMBO J. 1994 Apr 1;13(7):1514-24. Abdel-Meguid SS, Murthy HM, Steitz TA. Preliminary X-ray diffraction studies of the putative catalytic domain of gamma delta resolvase from Escherichia coli. J Biol Chem. 1986 Dec 5;261(34):15934-5. ---- Original message ---- >Date: Sat, 28 Jun 2008 18:30:38 -0400 >From: Gina Clayton <[EMAIL PROTECTED]> >Subject: [ccp4bb] Disordered domains in crystal strutures >To: [email protected] > >Dear CCP4ers > >can anyone recommend papers describing crystal structures of proteins >with a large functionally important disordered domain or domains. > >Thanks in advance > >Gina Phoebe A. Rice Assoc. Prof., Dept. of Biochemistry & Molecular Biology The University of Chicago phone 773 834 1723 http://bmb.bsd.uchicago.edu/Faculty_and_Research/01_Faculty/01_Faculty_Alphabetically.php?faculty_id=123 RNA is really nifty DNA is over fifty We have put them both in one book Please do take a really good look http://www.rsc.org/shop/books/2008/9780854042722.asp
