Subbu,
Take a look at the following for an example of how to proceed.
J. Ay et al., Proc. Natl. Acad. Sci. USAVol. 95, pp. 6613–6618, June
1998 Structure and function of the Bacillus hybrid enzyme GluXyn-1:
Native-like jellyroll fold preserved after insertion of autonomous
globular domain
Cheers,
Michael
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R. Michael Garavito, Ph.D.
Professor of Biochemistry & Molecular Biology
513 Biochemistry Bldg.
Michigan State University
East Lansing, MI 48824-1319
Office: (517) 355-9724 Lab: (517) 353-9125
FAX: (517) 353-9334 Email: [EMAIL PROTECTED]
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On Jul 1, 2008, at 6:29 AM, Narayanan Ramasubbu wrote:
Dear All:
I am working with two enzymes (40 kDa each). The crystal structures
of the two enzymes are known.
I have made a hybrid enzyme of the two individual ones.
This chimeric/hybrid enzyme is soluble and exhibits activity of the
two individual enzymes.
My questions are as follows:
1. Could I use one of the enzyme's structure to solve the crystal
structure of the hybrid by MR?
2. Has anyone working with such hybrids (?) noticed any increase in
specific activity of either of the enzymes?
Interestingly, the two enzymes work on the same substrate polymer.
One is a hydrolase another is an esterase.
Thanks a lot.
Subbu
