N-terminal sequencing / MS for intact mass analysis are the only ways that I can think of.....
Matt 2008/7/1 Klaus Futterer <[EMAIL PROTECTED]>: > We have a 150 kDa protein that reproducibly crystallises at one of the > Hampton Screen conditions. However, we know from SDS gel analysis that the > crystals contain only a 45 kDa fragment, that forms through proteolytic > cleavage over time. We would like to determine the sequence boundaries of > the fragment. > > We believe a combination of N-terminal sequencing plus MS analysis might > give us the information we need, but I was wondering whether the ccp4bb > community has other suggestions. > > Klaus > > > > > > > --------------------------------------------------------------------- > > Klaus Fütterer, Ph.D. > > School of Biosciences P: +44-(0)-121-414 5895 > University of Birmingham F: +44-(0)-121-414 5925 > Edgbaston E: [EMAIL PROTECTED] > Birmingham, B15 2TT, UK W: www.biochemistry.bham.ac.uk/klaus/ > --------------------------------------------------------------------- > ---------------------------------------------------------------------------- Matthew LH Chu PhD Student School of Pharmacy and Pharmaceutical Sciences University of Manchester ----------------------------------------------------------------------------
