Use the 15 minute protocol from the Hampton Research catalogue/website. They're normally quite small and easy to cryoprtect with a little glycerol. Size can also be varied by playing with the salt concentration (slightly higher [NaCl] giving more but smaller xtals)
 
Paul..
 
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Dr. Paul A. McEwan
Protein Crystallography Group
Office C58
Centre for Biomolecular Science
University of Nottingham
Nottingham
NG7 2RD
UK
Tel: 0115 8232018
http://www.nottingham.ac.uk/pharmacy/research/medicinal-chemistry-structural-biology/structbio.php
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