Hi all, a common issue with membrane protein strustures is that at the end of refinemnt, there are (almost) always blobs of unassigned density covering the hydrophobic region that can't be built (because they are too small for a complete detergent molecule for example). Would it be feasible/allowed to build individual methyl/methylene groups in those blobs, much like is done for waters? Within CNS, what topology/parameter files would have to be used?
I seem to recollect a fairly recent membrane protein structure paper that introduced something like this, but for the life of me I can't remember the reference. Cheers, Bert Bert van den Berg University of Massachusetts Medical School Program in Molecular Medicine Biotech II, 373 Plantation Street, Suite 115 Worcester MA 01605 Phone: 508 856 1201 (office); 508 856 1211 (lab) e-mail: [EMAIL PROTECTED] http://www.umassmed.edu/pmm/faculty/vandenberg.cfm
