Dear all,
I repeated a protein crystallization which is reported in PDB with the
almost same condition, and got a 2.6A data. But the problem is that I can
not determine the right phases with neither CCP4 nor CNS.
Then I found the protein in my crystal had been degraded from ~30kD to ~20kD
by SDS-PAGE, but did not know from which terminus it was truncated.
So I used truncated PDB templates which N-, C- or both terminal were cut to
fit the length of my shorter protein, and tried many different templates.
But always high R-factor (~0.5).
With COOT I found the backbone did not fill the electron density map
perfectly.
The only difference of my crystallization condition is 277K(mine) vs
295K(reported) in temprature.
Any suggestions will be appreciated.
Haitao ZHANG, Ph.D Student,
Shanghai Institute of Materia Medica,
Chinese Academy of Sciences
