Florian,
You can use additional planarity restraints for individual base pairs
for low resolution refinement. Easiest way to do this is to define
explicitly individual base pairs and use appropriate weights. You can
include the definitions in the 'dna-rna_restraints.def' file after the
'planarity-restraints' statement.
best,
Daniel
On Jul 8, 2008, at 1:00 AM, CCP4BB automatic digest system wrote:
does anyone know how to change the weighting of the Watson-Crick base
pair restraints using the dna-rna_restraints.def restraints definition
file in CNS? We are working with rather low resolution structures
and I
would like the restraints to be a bit tighter than they are by
default.
Thanks,
--
Daniel Panne, PhD
EMBL Group leader
Structure and Function of Macromolecular Assemblies
[EMAIL PROTECTED]
Tel: +33 476 207925
Fax: +33 476 207199
Web: http://www.embl.fr
Postal address: EMBL, 6 Rue Jules Horowitz, BP181, 38042 Grenoble
Cedex
9, France
