Defining specific restraints on bonds connecting the chromophore with the rest of the protein worked fine for us in Refmac when we solved a turboGFP structure a couple of years ago. There wasn’t anything special that had to be done �C all strictly by the book…
Artem _____ From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of sheng CUI Sent: Friday, August 01, 2008 7:32 AM To: [email protected] Subject: [ccp4bb] chromophore in GFP Hi everybody, we have solved the X-ray structure of a GFP (green fluorescent protein) containing complex. During refinement of the structure we now encountered the problem of connecting the GFP chromophore covalently to the polypeptide chain. We introduced the chromophore to our model as a separate ligand with separate toplogy. So far we could not find a way to integrate it in the primary sequence. Any idea how this problem can be solved? Which programs might be useful? Thank you very much in advance. Best, Sheng & Axel ************************************************************************* Dr. Sheng CUI 崔胜 Department of Chemistry and Biochemistry Gene Center University of Munich Feodor-Lynen-Str. 25 81377 Munich, Germany Tel: +49 89 2180-76984 Fax: +49 89 2180-76999 e-mail: <mailto:[EMAIL PROTECTED]> [EMAIL PROTECTED] Web: <http://www.lmb.uni-muenchen.de/hopfner/welcome.html> http://www.lmb.uni-muenchen.de/hopfner/welcome.html ***************************************************************************
