Dear Mark Most easy way to have relative estimation is to run PAGE of your protein with known proteins of known different concentration so u can have a good relative estimation.
but for more accurate estimation u can go for Biochemical methods such as Lowry method or BCA method which are more senstive then UV spectro method although they depend upon oxidation of total aromatic amino acid in protein u can try or wait for other suggestions.# . Puneet Juneja On Thu, Aug 21, 2008 at 12:54 PM, Mark Hilge <[EMAIL PROTECTED]> wrote: > Dear all, > > I would be glad to hear what (simple) method I should use to determine > protein concentrations as accurately as possible. Presently, I'm measuring > absorption at 280nm with a nanodrop device. I either have 0 or 1 tryptophan > and no activity test. > > Many thanks in advance! > > Best regards, > > Mark > > Mark Hilge > Protein Biophysics > NCMLS 274 > 3rd floor M850.03.035 > Geert Grooteplein 28 > 6525 GA Nijmegen > The Netherlands > > http://www.mark-hilge.com > > Phone: 0031 24 36 10 525 > > > > Het UMC St Radboud staat geregistreerd bij de Kamer van Koophandel in het > handelsregister onder nummer 41055629. > The Radboud University Nijmegen Medical Centre is listed in the Commercial > Register of the Chamber of Commerce under file number 41055629. >
