Exactly. There are about fifteen reasons that I could name without thinking as to why any particular step in restriction-based cloning may not work. If I think about it the number goes up to thirty or more. Yet only ONE of them is what actually plagues you. So please Vijay, give us sufficient details to suggest something reasonable.
Artem -----Original Message----- From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Frank von Delft Sent: Saturday, August 30, 2008 7:24 AM To: [email protected] Subject: Re: [ccp4bb] hi PS. Sorry, couldn't resist. But seriously, for those new to the list: if you want someone to spend time writing a useful answer, then you need to spend time formulating a useful question. Good: specific questions with sufficient background given Bad: general questions which are best answered by (a) a textbook/manual or (b) doing a PhD on it. phx. vijay srivastava wrote: > hi > i am facing problem in cloning,getting my insert and vector at the > correct position after digestion but after ligation colony is not > coming and if how it is coming than i am not getting my insert > > > > ------------------------------------------------------------------------ > Unlimited freedom, unlimited storage. Get it now > <http://in.rd.yahoo.com/tagline_mail_2/*http://help.yahoo.com/l/in/yahoo/mai l/yahoomail/tools/tools-08.html/>
