If your peptide has less than 400 atoms, you can also try and use the 'Fit Ligand' option under the "Calculate" menu of the main window: read in the coordinates of a peptide of the same length as the one you wish and use "Simple Mutate" from the "Model/Fit/Refine" menu to adjust to the desired sequence. Then choose "fit ligand" and click "flexible". That often works amazingly well with decent density.

Tim

--
Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen

GPG Key ID = A46BEE1A


On Tue, 2 Sep 2008, Tarique wrote:

Hi,
Can any body tell me how to build peptide fragment of 5-6 residues using
coot,if the sequence is known.I am working at 2.1 angstroms resolution map.

Tarique Khan
Research scholar
Structural Bilogy Unit,
National institute of Immunology,
Aruna Asaf Ali marg,
new Delhi 110067
India
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