Hi Matt, to check, if the color comes from a metal ion, you can get an AAS analysis done. You need something in the range of 1 mg of well dialyzed protein (metal free buffer!). We got an analysis done for the most important metals (Mn, Fe, Co, Ni, Cu, Zn) for ca. 20 Eur per element from a local analytic company. greetings Gottfried
Dr. Gottfried Palm Ernst-Moritz-Arndt-Universität Inst. für Biochemie (MNF) Abt. Biochemie I Felix-Hausdorff-Straße 4 17489 Greifswald Tel. +49(03834)-864393 Fax. +49(03834)-864373 email [EMAIL PROTECTED] =================================================================== WEB-Mailer der Uni-Greifswald ( http://www.uni-greifswald.de/ ) ===================================================================