Dear Rajakumara I forgot to say, there's a very simple way to do this by "aliasing".
You write (let's say by hand) one or more random screens with solutions called e.g. Precipitate1, Precipitate2, .... PrecipitateN; Buffer1 to BufferN; Additive1 to AdditiveN etc. Each time you want to make a screen you simply replace Precipitate1, Precipitate2 etc. with the precipitates you want to use, say 30% PEG, 2M AS etc. It's almost trivial to do the whole thing in Excel using a look-up table - or just links If you have a robot you can run the same script each time, just put the appropriate solutions into the stock wells. Patrick -- For information and discussion about protein crystallization and automation, please join our bulletin board at http://groups.google.com/group/oryx_group/members_invite?hl=en [EMAIL PROTECTED] Douglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090 US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36 > -----Original Message----- > From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of E > rajakumar > Sent: 12 September 2008 15:17 > To: CCP4BB@JISCMAIL.AC.UK > Subject: [ccp4bb] Query on program for creating random crystallization > screen > > Dear All > I want to create a random crystallization screen using > given set of crystallization parameters (pH, > Precipitant, salt, additive.....) for protein-DNA > complex crystallization. I was using Brent Segelke's > CRYSTOOL program, not accessible online now. Please > can you suggest any other such programs and/ how do I > can access the CRYSTOOL (sort of registration or > purchase?) > > Thanking you in advance > Rajakumara > > > E. Rajakumara > Postdoctoral Fellow > Strcutural Biology Program > Memorial Sloan-Kettering Cancer Center > New York-10021 > NY > 001 212 639 7986 (Lab) > 001 917 674 6266 (Mobile) > > > > Get your preferred Email name! > Now you can @ymail.com and @rocketmail.com. > http://mail.promotions.yahoo.com/newdomains/aa/
