Hello all,

I have a structure from a non-natural fusion of the truncated C- terminus of one protein with the truncated N-terminus of another. For the deposition, we want to keep the numbering as found in the separate proteins. It looks something like this:

              1         12
              |          |
....HWVCKDIALLMCFFLEEMSEEP....
    |        |
  754      763

At no point is there an overlap in numbering (i.e. the N-terminal residue number is higher than the C-terminal residue number).

Is this numbering scheme supported by the PDB standard? Thus far, all of the software seems to handle it (refmac, Coot, PyMOL, pdb_extract, PDB precheck & validation, etc).

Can anyone see a reason to not deposit with this non-sequential residue numbering?

~Todd

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