what is it that you want to demonstrate? If it is just for structure
solution, would S-SAD phasing be an option? That way all you need is a
crystal, probably diffracting to better than, say, 2A. And a little bit of
multiplicity.
I assume this is feasible for Lysozyme whereas I don't know the
diffraction qualities of proteinase K crystals.
Tim
--
Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen
GPG Key ID = A46BEE1A
On Tue, 23 Sep 2008, Tommi Kajander wrote:
Hi,
Could anyone give a more or less exact recipee for preparing a derivate for
lysozyme or proteinase K?
like what used, concentration and soak time. we'd need some data sets for a
lab course only thing that
really worked so far was lysozyem KI SIRAS (which however doesnt seem to be
good enough for demonstration
purposes, so we need more)
Thanks very much!
best,
Tommi
Tommi Kajander, Ph.D.
Structural Biology and Biophysics
Institute of Biotechnology
University of Helsinki
Viikinkaari 1
(P.O. Box 65)
00014 Helsinki
Finland
p. +358-9-19158903
[EMAIL PROTECTED]
