Hi Rongjin, I had crystals that grew in 9% ethanol as a precipitant. To cryoprotect them, I first replaced the mother liquor with solution containing 10% MPD, instead of ethanol, and then briefly dunked the crystals into ~30% MPD.
Hope this helps. Good luck. Katya On Mon, Sep 29, 2008 at 1:24 PM, Rongjin Guan <[EMAIL PROTECTED]> wrote: > Dear All > > I got crystals from 20% Ethanol with 0.1M Tris pH 8.5. This is my first > time to have crystals in Ethanol and want to get some suggestions of > cryo-protection from those who have done this before. > > I am waiting for my time on home X-ray facility, and hope I can get > some suggestions before that. > > Thanks, > > Rongjin Guan >
