You could also try seeding, crush up your needles seed a new plate and
re-screen. With re-screen I mean not your original conditions but e.g.
Hampton 1&2 or orthers and see if you get a different condition with
other crystals.
You might also see into your current condition. I assume you have
screened for pH already (not only using the buffer which is in your
current condition).
Good luck,
Jürgen
P.S. Besides are the crystals mountable and >5 µm thick why don't you
go to SLS or ESRF and collect data there ? Thick does not necessarily
mean better.
On 14 Oct 2008, at 04:09, shivesh kumar wrote:
Dear all,
I have crystallized a protein in MPD which is thin and growing in
one direction only. I have tried ethonal, DMSO, Proline, Glucose,
Urea, Sucrose, Detergent kit from Hampton and lot more alongwith
temperature variation. Need suggestions regarding improvement of
xtal quality, THICKNESS.
Thanx in advance.
Shivesh
-
Jürgen Bosch
University of Washington
Dept. of Biochemistry, K-426
1705 NE Pacific Street
Seattle, WA 98195
Box 357742
Phone: +1-206-616-4510
FAX: +1-206-685-7002
Web: http://faculty.washington.edu/jbosch
