Hi, all: I have a poor density after phase extension problem. Usually I use HKL2000 to scale the data and use the macro at the integrate and scale steps. I got a good native data( P6422 130 130 150 , 90 90 120) at 3.0A (completeness >90%, I/sigma >5, redandance>10, Linear R-fac<0.5, but square R-fac >0.6; one problem is Linear R-fac smaller than squear R-fac in my scaled data. I used macro: sigma cutoff 0 during scaling stage. if i don't use macro, the data will be getting bad less than 4.0A) and my reflection data has strong anisotropy.
I used this native data to do phase extension from 3.6A to 3.0A by CCP4i DM program(the phase got from sharp program), but I cannot find any density is improved.(density of sidechain cannot be seen) my question is what happened in my data? the data qaulity is enough to see the density of sidechain. How i can improve the density? Any comment appreciated! thank you joe
