Dear all,
I received several replies to my original question, then a little
hiatus, then I made the Summary. Following the summary, some more
contributions to my original question came. So, I would like to add to
the summary:
- Do not care about tag names, common sense is to care not to have a
long extenstion. Consider carboxypeptidases to remove C-terminal tags,
eventual heterogeneity might not hurt.
- Consider BioRad new kit for producing tag-less very pure protein.
Great with proteins of compromised solubility or low expression.
- Tags effects highly dependent on the protein, etc. Again, might or
might not help crystallization. Two experiences with c-Myc tags , no
crystals with or without, other approach needed (the cost of the resin
to be considered).
Thanks again for all comments.
Jorge
