Dear All, I am trying to co-crystallize a protein with substrate/substrate analogue and for some reason it is not working. I have tried to co-crystallize with upto 2 mM substrate and it does not bind. The protein precipitates out if I go higher concentration. Soaking does not work either. Is there any suggestion that I should try before I give up on this.
Thanks a lot. Priya -- Priya Mudgal PhD Student Duke University
