You might be right. I can not rule out the possibility. By the way, is the PMS/DCPIP system fairly stable in the aerobic condition?
Mike --- On Thu, 12/4/08, [EMAIL PROTECTED] <[EMAIL PROTECTED]> wrote: From: [EMAIL PROTECTED] <[EMAIL PROTECTED]> Subject: Re: [ccp4bb] Offtopic: FAD enzymatic assay To: [EMAIL PROTECTED] Date: Thursday, December 4, 2008, 5:37 PM CCP4 bulletin board <[email protected]> wrote on 12/04/2008 06:21:55 PM: > Dear all, > > I may sound stupid enough. > > I tried the PMS-DCPIP assay system as suggested, and I choose to > observe the absorption at 600nM. However, when I initialize the > reaction, I actually see the Abs slowly but steadily increasing > rather than decreasing. > When I used to run enzymatic assays similar to this (NADH-coupled assays), I would also see a slow, steady increase in absorbance even in the absence of enzymatic activity. I attributed it to evaporation of water from the (open, incubated at 37 C) cuvette causing a gradual increase in concentration of the reagents. Is this possible here? - Matt -- Matthew Franklin , Ph.D. Senior Scientist, ImClone Systems 180 Varick Street, 6th floor New York, NY 10014 phone:(917)606-4116 fax:(212)645-2054 Confidentiality Note: This e-mail, and any attachment to it, contains privileged and confidential information intended only for the use of the individual(s) or entity named on the e-mail. If the reader of this e-mail is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that reading it is strictly prohibited. If you have received this e-mail in error, please immediately return it to the sender and delete it from your system. Thank you.
