Hi Somu, Here are a few questions which might help: Are you sure about about your space group? Is another lattice/symmetry possible? Is twinning possible?
Have you tried all possible space groups in MR? Have you tried different resolution ranges (e.g. all data, cut low resolution at 15 A, cut high resolution at 3 or 4 A)? Do you have models from other homologs? You can try them individually or input everything you have in phaser. Did you input 76% sequence identity in phaser? Try with lower values (larger difference between MR model and structure) or higher rmsd values (1.0, 1.2, 1.5) instead. Good luck, christian Meetmr Ss wrote: > Dear all, > > I struck with MR problem. MY target has 76% sequence identity with the > model. I tried Phaser, AMoRe and Molrep. None of them gave me > satisfactory solution. If you have any suggestions and or New programs I > would like to try. > > Thanks > somu > _______________________________________________________________________ Dr. Christian Biertümpfel Laboratory of Molecular Biology NIDDK/National Institutes of Health phone: +1 301 402 4647 9000 Rockville Pike, Bldg. 5, Rm. B1-03 fax: +1 301 496 0201 Bethesda, MD 20892-0580 USA _______________________________________________________________________
