[ccp4bb] ultrafiltration/buffer exchange device for insect cell cultures

[Michael Hothorn]

Dear all,

sorry for the non-CCP4 question. I am expressing a protein in insect 
cells that is targeted for secretion into the medium. After expression, 
I want to concentrate the medium containing my protein of interest (from 
10 l to 0.3 L) and at the same time change the buffer to something that 
is compatible with my first ion exchange chromatography step. I would be 
interested to know what kind of ultrafiltration devices can be used for 
this purpose, and whether some of you could suggest a quick, reliable 
and efficient system that we could purchase for our lab.

Thanks!
Michael