Are the crystals growing as a result of the skin, or coincidentally located with the skin? For instance, if you are using the hanging drop method, and the crystals are growing with the skin because of their density, you could switch to sitting drop plates -- crystals grow on the bottom, skin stays on top. If the skin is required for crystal growth, then of course this doesn't apply. Generally, this is also simpler than converting the growth conditions from VD to batch under oil.
-Jesse On Tue, Dec 30, 2008 at 11:22 AM, riya doreen <driy...@gmail.com> wrote: > Hello Everyone, > > Thank you for all the suggestions on how to manipulate skin on crystal > drops. Here is a summary of responses: > > (i) Higher concentrations of reducing agents (ii) Layering crystal drop > with cryo-protectant (iii) Trypsinize the skin (iv) Grow crystals under oil > (v) Try 4C if crystals are grown at 18 or RT (vi) Lower protein > concentration and (vii) Fiddling around with the drop which may break the > skin and free the crystals > > > On Sun, Dec 28, 2008 at 8:24 PM, riya doreen <driy...@gmail.com> wrote: > >> *Hello All, >> >> I am trying to optimize protein crystals that grow from skin. All efforts >> to separate crystals from skin have failed since it is fairly thick. >> Crystallization under oil eliminates skin but the resultant crystals are >> very thin and small needles. >> >> I would appreciate any suggestions on how this problem can be eliminated. >> >> Thanks >> >> * > > >
